DNA

Part:BBa_K4663027:Design

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


Barcode construct for P. fluorescens knock-in


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 245
    Illegal NgoMIV site found at 299
    Illegal NgoMIV site found at 699
    Illegal AgeI site found at 1058
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 847
    Illegal BsaI.rc site found at 490


Design Notes

None


Source

None

References